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Laboratory | Epigenetics | Brain | Alzheimer's disease | Cognition | Genomics


LEM

Mammalian Epigenomics Laboratory

​ FRENCH

Published on 25 January 2023
Accurate control of genome expression is crucial for proper cell functioning. Our team is investigating how the epigenetic regulation of gene expression is altered in the brain during aging and Alzheimer’s disease, with the aim of identifying novel strategies for the treatment of cognitive impairment.

Team Leader

Matthieu GERARD
+33 1 69 08 94 29
Matthieu.Gerard@Cea.Fr
Mammalian Epigenomics (Matthieu GÉRARD)

 

​

Our team is expert in the study of the mechanisms that regulate gene expression and access of the transcription machinery to the genome in its physiological chromatin environment (e.g. Houlard et al., 2006, PMID: 17083276; Chantalat et al., 2011, PMID: 21803857; Carrière et al., 2012, PMID: 21911356; Montellier et al., 2013, PMID: 23884607). The basic unit of chromatin, the nucleosome, is composed of 147 base pairs of DNA tightly wrapped around a core of eight histone proteins. Nucleosomal organization represents a strong physical barrier that prevents binding of most transcription factors to DNA. ATP-dependent chromatin remodeling factors (also called remodelers) are enzymes that control the density and positioning of nucleosomes on the genome. A major predicted function of these enzymes is to regulate access of the transcription machinery to the genome by generating (or suppressing) open chromatin regions at specific locations. 

​Human resources

Matthieu GÉRARD, Researcher Emilie DROUINEAU, Research Engineer
Hélène PICAUD, Technician
Anida MESIHOVIC, Post doctoral fellow
Marina NOCENTE, PhD Student

RNA interference platform (PARi)

 (is part of the lab)

Scientific management
Matthieu GÉRARD (Researcher, CEA)
Operational management
Guillaume PINNA (Researcher, CEA)
Gueorgui KRATASSIOUK (Research Ingeneer, CNRS)
Marie VANDAMME (Technician, CEA)
Bioinformatic
Nadya MOROZOVA (Research Ingeneer, CNRS)

​Gene targeting and animal facility

Patrick HERY, Technician, Platform manager
+33 1 69 08 94 32
patrick.hery@cea.fr

Anne-Sophie CHAPLAULT, Technician
+33 1 69 08 80 18
anne-sophie.chaplaut@cea.fr

Sylvain THESSIER, Technician
+33 1 69 08 80 18
sylvain.thessier@cea.fr

Jean-Charles ROBILLARD, Technician
+33 1 69 08 54 78
jean-charles.robillard@cea.fr

 

​Publications
Publications HAL de Matthieu, GÉRARD ; Guillaume, PINNA de la structure I2BC

2022

Journal articles

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Emeline Fontaine, Christophe Papin, Guillaume Martinez, Stéphanie Le Gras, Roland Abi Nahed, et al.. Dual role of histone variant H3.3B in spermatogenesis: positive regulation of piRNA transcription and implication in X-chromosome inactivation. Nucleic Acids Research, 2022, 50 (13), pp.7350-7366. ⟨10.1093/nar/gkac541⟩. ⟨hal-03852700⟩
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https://hal.science/hal-03852700/file/gkac541.pdf BibTex

2021

Journal articles

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Valentin L’hôte, Régis Courbeyrette, Guillaume Pinna, Jean‐christophe Cintrat, Gwenaëlle Le Pavec, et al.. Ouabain and chloroquine trigger senolysis of BRAF‐V600E‐induced senescent cells by targeting autophagy. Aging Cell, 2021, 20 (9), pp.acel.13447. ⟨10.1111/acel.13447⟩. ⟨cea-04352688⟩
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https://cea.hal.science/cea-04352688/file/ACEL-20-e13447.pdf BibTex
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Lucie Jamgotchian, Solenne Vaillant, Erwan Selingue, Anaelle Doerflinger, Agathe Belime, et al.. Tumor-targeted superfluorinated micellar probe for sensitive in vivo 19 F-MRI. Nanoscale, 2021, 13 (4), pp.2373-2377. ⟨10.1039/d0nr08200g⟩. ⟨hal-03184962⟩
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2020

Journal articles

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Emilie Lebraud, Guillaume Pinna, Capucine Siberchicot, Jordane Dépagne, Didier Busso, et al.. Chromatin recruitment of OGG1 requires cohesin and mediator and is essential for efficient 8-oxoG removal. Nucleic Acids Research, 2020, ⟨10.1093/nar/gkaa611⟩. ⟨hal-02909621⟩
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Fatemeh Rajabi, Win-Yan Liu-Bordes, Marina Pinskaya, Foretek Dominika, Gueorgui Kratassiouk, et al.. CPEB1 orchestrates a fine-tuning of miR-145-5p tumor-suppressive activity on TWIST1 translation in prostate cancer cells. Oncotarget, 2020, 11 (45), pp.4155--4168. ⟨10.18632/oncotarget.27806⟩. ⟨hal-03031720⟩
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Elodie Villa, Rachel Paul, Ophélie Meynet, Sophie Volturo, Guillaume Pinna, et al.. The E3 ligase UBR2 regulates cell death under caspase deficiency via Erk/MAPK pathway. Cell Death and Disease, 2020, 11 (12), pp.1041. ⟨10.1038/s41419-020-03258-3⟩. ⟨hal-03102334⟩
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Natalia Rubanova, Guillaume Pinna, Jeremie Kropp, Anna Campalans, Juan Pablo Radicella, et al.. MasterPATH: network analysis of functional genomics screening data. BMC Genomics, 2020, 21 (1), pp.632. ⟨10.1186/s12864-020-07047-2⟩. ⟨hal-02944249⟩
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https://hal.science/hal-02944249/file/12864_2020_Article_7047.pdf BibTex
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Pierre Giroux, Ricky Bhajun, Stéphane Segard, Claire Picquenot, Céline Charavay, et al.. miRViz: a novel webserver application to visualize and interpret microRNA datasets. Nucleic Acids Research, 2020, ⟨10.1093/nar/gkaa259⟩. ⟨hal-02557471⟩
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https://hal.science/hal-02557471/file/Giroux_Guyon_NAR_2020_miRViz.pdf BibTex

2019

Journal articles

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May Bakail, Albane Gaubert, Jessica Andreani, Gwenaëlle Moal, Guillaume Pinna, et al.. Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1. Cell Chemical Biology, 2019, 26 (11), pp.1573-1585.e10. ⟨10.1016/j.chembiol.2019.09.002⟩. ⟨hal-02385251⟩
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https://hal.science/hal-02385251/file/Bakail%20M.%20et%20al%202019.pdf BibTex
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Cyril Carvalho, Valentin L'Hôte, Régis Courbeyrette, Gueorgui Kratassiouk, Guillaume Pinna, et al.. Glucocorticoids delay RAF-induced senescence promoted by EGR1. Journal of Cell Science, 2019, 132 (16), pp.jcs230748. ⟨10.1242/jcs.230748⟩. ⟨hal-02330078⟩
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Minh-Duc Hoang, Marie Vandamme, Gueorgui Kratassiouk, Guillaume Pinna, Edmond Gravel, et al.. Tuning the cationic interface of simple polydiacetylene micelles to improve siRNA delivery at the cellular level. Nanoscale Advances, 2019, 1 (11), pp.4331-4338. ⟨10.1039/C9NA00571D⟩. ⟨cea-04492443⟩
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Minh-Duc Hoang, Marie Vandamme, Gueorgui Kratassiouk, Guillaume Pinna, Edmond Gravel, et al.. Tuning the cationic interface of simple polydiacetylene micelles to improve siRNA delivery at the cellular level. Nanoscale Advances, 2019, 1 (11), pp.4331--4338. ⟨10.1039/C9NA00571D⟩. ⟨hal-02389246⟩
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Abir Arfaoui, Claire Rioualen, Violette Azzoni, Guillaume Pinna, Pascal Finetti, et al.. A genome-wide RNAi screen reveals essential therapeutic targets of breast cancer stem cells. EMBO Molecular Medicine, 2019, 11 (10), pp.e9930. ⟨10.15252/emmm.201809930⟩. ⟨hal-02539524⟩
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https://hal.science/hal-02539524/file/Arfaoui%20et%20al.%20%28RNAi%20screen%29.pdf BibTex
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Laurent Suissa, Virginie Flachon, Jean-Marie Guigonis, Charles-Vivien Olivieri, Fanny Burel-Vandenbos, et al.. Urinary ketone body loss leads to degeneration of brain white matter in elderly SLC5A8-deficient mice. Journal of Cerebral Blood Flow and Metabolism, 2019, pp.271678X19873662. ⟨10.1177/0271678X19873662⟩. ⟨hal-02294233⟩
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Nikolay Bessonov, Guillaume Pinna, Andrey Minarsky, Annick Harel-Bellan, Nadya Morozova. Mathematical modeling reveals the factors involved in the phenomena of cancer stem cells stabilization. PLoS ONE, 2019, 14 (11), pp.e0224787. ⟨10.1371/journal.pone.0224787⟩. ⟨hal-02367567⟩
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2018

Journal articles

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Soizik Berlivet, Virginie Guiraud, Martin Houlard, Matthieu Gérard. pHYPER, a shRNA Vector for High-Efficiency RNA Interference in Embryonic Stem Cells. Biotechniques, 2018, 42 (6), pp.738-743. ⟨10.2144/000112454⟩. ⟨cea-04847960⟩
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Murli Mishra, Hong Jiang, Hedy A. Chawsheen, Matthieu Gérard, Michel B. Toledano, et al.. Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis. Cancer Letters, 2018, 432, pp.216--226. ⟨10.1016/j.canlet.2018.06.011⟩. ⟨hal-02182306⟩
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Anaëlle Doerflinger, Nam Nguyen Quang, Edmond Gravel, Guillaume Pinna, Marie Vandamme, et al.. Biotin-functionalized targeted polydiacetylene micelles. Chemical Communications, 2018, ⟨10.1039/C8CC00553B⟩. ⟨hal-02177608⟩
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