Know-how
- Cell Analysis
- Analysis of heterogeneous samples of fluorescently-labeled particles.
- Multi-laser configurations for multi-color applications (5 lasers/up to 40 colors).
- Autonomous use by researchers approved by the platform.
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- Separation of cells or particles of interest by electrostatic deflection. Collection of up to 4 fractions per sample.
- Aria Sorp FACS sorter. Autonomous sorting by teams.
- High-speed Influx sorter (up to 50,000 events per second) for selection of rare subpopulations (< 0.01%; e.g. stem cells). Sorting carried out by the Platform in collaboration with the teams.
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Applications and projects
- Research and Development
- Analysis of biological samples (e.g. blood, bone marrow, cell culture).
- Sorting of cell populations for analysis (Single Cell, RNA or DNA, transplantation, culture).
Analysis and sorting of hematopoietic, neural and gonadal stem cells for various research programs. Applications in radiobiology, oncology, regenerative medicine (stem cell transplantation for tissue or organ reconstitution).
- Intrinsic and hereditary radiosensitivity, through analysis of radiation-induced apoptosis and transcriptome in lymphocyte subpopulations of related individuals from large families.