Céline AMIEZ, CNRS Stem Cell and Brain Research Institute, Inserm, University Lyon 1 has given a talk on Zoom on November 28th.

https://sbri.fr/

Short abstract:

Custom-address serial holography (CASH) is a new method based on fast acousto-optic modulation for optical recording of neuronal activity in 3D at high speed in-vivo. CASH allows random address sampling of 20 cells at 1 kHz up to 200 cells at 0.1 kHz in head-fixed behaving mice across a volume of 500 µm3. 3D-CASH recordings of GCaMP6f expressing neurons in layer 2/3 and 5 of mouse primary visual cortex in response to moving contrast gratings reveal the layered organization of the cortex, in the structure of the neuron pairwise correlation matrix. Following the stimulus temporal periodicity, the response spike rate features phasic (R1) and non-phasic component (R0); R1/R0 values show a weak bimodality resembling the transition between non-phasic neurons to phasic neurons with complex and simple receptive fields. Our data validate 3D-CASH as a method for assessing neuronal activity in 3D-distributed cortical circuits at high sampling rate.