You are here : Home > Cerebrospinal fluid lens-free microscopy: A new tool for the laboratory diagnosis of meningitis

Publications

Cerebrospinal fluid lens-free microscopy: A new tool for the laboratory diagnosis of meningitis

Published on 29 March 2018
Cerebrospinal fluid lens-free microscopy: A new tool for the laboratory diagnosis of meningitis
Description
 
Date 
Authors
Delacroix R., Morel S.N.A., Hervé L., Bordy T., Dinten J.-M., Drancourt M., Allier C.
Year2017-0086
Source-TitleScientific Reports
Affiliations
Aix Marseille Univ, INSERM, CNRS, IRD, URMITE, Marseille, France, Université de Grenoble Alpes, Grenoble, France, CEA, LETI, MINATEC Campus, Technologies for Healthcare and Biology Division, Grenoble, France
Abstract
Cerebrospinal fluid cytology is performed by operator-dependant light microscopy as part of the routine laboratory work-flow diagnosis of meningitis. We evaluated operator-independent lens-free microscopy numeration of erythrocytes and leukocytes for the cytological diagnosis of meningitis. In a first step, prospective optical microscopy counts of leukocytes done by five different operators yielded an overall 16.7% misclassification of 72 cerebrospinal fluid specimens in meningitis/non-meningitis categories using a 10 leukocyte/?L cut-off. In a second step, the lens-free microscopy algorithm adapted for counting cerebrospinal fluid cells and discriminating leukocytes from erythrocytes was modified step-by-step in the prospective analysis of 215 cerebrospinal fluid specimens. The definite algorithm yielded a 100% sensitivity and a 86% specificity compared to confirmed diagnostics. In a third step, a blind lens-free microscopic analysis of 116 cerebrospinal fluid specimens, including six cases of microbiology-confirmed infectious meningitis, yielded a 100% sensitivity and a 79% specificity. Adapted lens-free microscopy is thus emerging as an operator-independent technique for the rapid numeration of leukocytes and erythrocytes in cerebrospinal fluid. In particular, this technique is well suited to the rapid diagnosis of meningitis at point-of-care laboratories. © The Author(s) 2017.
Author-Keywords
 
Index-Keywords
 
ISSN20452322
LinkLink

Go back to list