University of Grenoble Alpes, CNRS, Grenoble INP, LGP2, Grenoble, France, CEA, LETI, MINATEC Campus, Grenoble, France

Nucleic acid amplification testing is a very powerful method to perform efficient and early diagnostics. However, the integration of a DNA amplification reaction with its associated detection in a low-cost, portable, and autonomous device remains challenging. Addressing this challenge, the use of screen-printed electrochemical sensor is reported. To achieve the detection of the DNA amplification reaction, a real-time monitoring of the hydronium ions concentration, a byproduct of this reaction, is performed. Such measurements are done by potentiometry using polyaniline (PAni)-based working electrodes and silver/silver chloride reference electrodes. The developed potentiometric sensor is shown to enable the real-time monitoring of a loop-mediated isothermal amplification (LAMP) reaction with an initial number of DNA strands as low as 10 copies. In addition, the performance of this PAni-based sensor is compared to fluorescence measurements, and it is shown that similar results are obtained for both methods. © 2017 American Chemical Society.

DNA, Electrodes, Isotherms, Nucleic acids, Polyaniline, Potentiometers (electric measuring instruments), Autonomous devices, DNA amplification, Fluorescence measurements, Loop mediated isothermal amplifications, Polyanilines (PAni), Real time monitoring, Reference electrodes, Working electrode, Electrochemical sensors