Phage display nanobody selection from our fully synthetic humanized library [1] is a reality with more than a hundred successful projects since the publication of our library [2-7]. To complete our phage display selection of nanobodies, we took advantage from our 20 years of experience in Yeast genetics and developed both a Yeast 2-Hybrid platform and a Yeast display platform for optimization and intrabodization of nanobodies coming from our library or from the lama [7]. The selection and optimization of nanobodies and specifically nanobodies that could be expressed inside the cells (Intrabodies) will be illustrated with Nanobodies directed against TAU and providing anti-aggregative activity in vitro, in cellulo and in vivo in a mouse model of tauopathy.

1) Moutel S. et al. Elife 2016, 19 : 5
2) Mikhaylova M, et al.Nat Commun., 2015 Aug 11;6:7933
3) Galli V, et al. Elife, 2017 Oct 12; 6. pii: e25197
4) Jang H, et al. Mol Immunol. 2020 Feb;118:110-116
5) Elorza-Vidal X et al. Hum Mol Genet. 2020 May 8;29(7):1107-1120
6) Kawecki C, et al. J Thromb Haemost. 2020 Jun 4.
7) Dupré E, et al. ACS Chem Neurosci. 2019 Sep 18;10(9):3997-4006

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